celloidin
From: | A van Binsbergen <anneb@mail.saimr.wits.ac.za> |
I used to routinely celloidinise(?sp) all my brain sections for silver
stains in celloidin
1% celloidin in ether alcohol (1:1)
sections from 100% alcohol into 1% celloidin for about 2mins
remove and drain upright until set to touch (try it and you'll know what I
mean)
harden in 70% alcohol - 1 min
rinse in running h2O
stain as desired
There will be some of you who pour the celloidin on and immediately drain it
off - fine for 1 or 2 slides
I also found that the celloidin (and section ) started peeling off the slide
if I used the pour on drain off method
Never had a wash off with my coplin jar technique!!
Annie in Africa
Anne S. van Binsbergen
S.A.I.M.R./Chris Hani Baragwanath Hospital
Johannesburg
South Africa
+2711 4898717 (fax)
+2711 4898711
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