bone staining question
|From:||"Smith, Erin" <Erin.Smith@smbio.com>|
I have a question about doing bone histology on GMA embedded mouse proximal
tibia (undecalcified). While these animals were alive, oxytetracycline was
administered sub-cutaneously. The company I contract for wants to see if
the Tet label is visible in the trabecular bone of these tibia. I have cut
the sections (frontal plane) and mounted them on slides, drying in a 140 C
Do I need to do a light counterstain? Or would that interfere with the
fluorescence of the Tetracycline? Any suggestions would be wonderful.
--fyi...I will be taking digital photographs of these slides using a
microscope that has a UV lamp.
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