bone staining question

From:"Smith, Erin" <>

Good Morning!

I have a question about doing bone histology on GMA embedded mouse proximal
tibia (undecalcified).  While these animals were alive, oxytetracycline was
administered sub-cutaneously.  The company I contract for wants to see if
the Tet label is visible in the trabecular bone of these tibia.  I have cut
the sections (frontal plane) and mounted them on slides, drying in a 140 C

Do I need to do a light counterstain?  Or would that interfere with the
fluorescence of the Tetracycline?  Any suggestions would be wonderful.



--fyi...I will be taking digital photographs of these slides using a
microscope that has a UV lamp.

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