Andrea,
The best thing to do is run your existing technique in parallel with the
modified using aqueous picric acid. Use mirror sections, possibly kidney,
liver, tongue and intestine, and compare the results. Record in the lab's
development notebook and that should satisfy the accreditors.
Regards
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Andrea Grantham <algranth@u.arizona.edu> 17/May/2001 06:27am
>>>
A few days ago there was a discussion about use of Bouins and John
Kiernan 
sent a post saying that it was not necessary to use Bouins (for the
Masson 
Trichrome) when a Sat aqueous picric acid solution works just as well.
Is 
this documented somewhere? If I change the written procedure I'd like to 
include a reference.
Andi
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html





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