Safranin-O staining on EDTA decalcified rat bone

From:"Kowsz, Kim P" <>

I have formalin fixed paraffin embedded rat bone that has been decalcified
in 14% EDTA.  I am looking at articular cartilage on the stifle joint.  When
we did our routine Safranin-O, the EDTA decalcified bone did not pick up the
Safranin-O.  Sections decalcified with formic acid and stained in the same
batch worked beautifully.  Does the EDTA bone need some sort of
pretreatment?  I have tried 1% magnesium chloride for 2 hours but this
didn't help.

Kim Kowsz

Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.

<< Previous Message | Next Message >>