Safranin-O staining on EDTA decalcified rat bone

From:"Kowsz, Kim P" <kim_p_kowsz@groton.pfizer.com>

I have formalin fixed paraffin embedded rat bone that has been decalcified
in 14% EDTA.  I am looking at articular cartilage on the stifle joint.  When
we did our routine Safranin-O, the EDTA decalcified bone did not pick up the
Safranin-O.  Sections decalcified with formic acid and stained in the same
batch worked beautifully.  Does the EDTA bone need some sort of
pretreatment?  I have tried 1% magnesium chloride for 2 hours but this
didn't help.

Kim Kowsz
kowszkp@groton.pfizer.com


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