Re Oil red staining
From: | RichardWHorobin@aol.com |
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Jaclynn Lett
wrote:
> Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
> (Epon or Epon-Araldite)? If so, has this been done by staining en bloc or
> by staining the sections. Sections would range from 1-4 microns in
> thickness.
A couple of people so far have commented on how hard this would be, and our
experience agrees with theirs.
HOWEVER Jaclynn also said:
> We would also consider tissues embedded in glycol methacrylate. We'd like
> to avoid frozen sections because we'd prefer the higher level of detail
> possible with plastic.
In THAT case things look better! Would you settle for Sudan black, rather
than Oil red staining of the lipid? If 'yes' then there is a method - which
does indeed show even tiny droplets of lipid very clearly. This was worked
out by the one-time king of GMA staining Peter Gerrits, and can be found in J
Neurosci Methods, as follows:
Gerrits PO, Brekelmans-Bartels M, Mast L, 's-GrAavenmade EJ, Horobin RW
and Holstege G. (1992)..
Staining myelin and myelin-like degradation products in the spinal
cords of chronic experimental
allergic encephalomyelitis (Cr-EAE) rats using Sudan Black B staining
of glycol methacrylate-embedded
material.
J. Neuroscience Methods. 45, 99-105
Bye - Richard Horobin
Institute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E RichardWHorobin@aol.com
"What should we expect? Everything."
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Jaclynn Lett
<BR>wrote:
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
<BR>(Epon or Epon-Araldite)? If so, has this been done by staining en bloc or
<BR>by staining the sections. Sections would range from 1-4 microns in
<BR>thickness. </BLOCKQUOTE>
<BR>
<BR>A couple of people so far have commented on how hard this would be, and our
<BR>experience agrees with theirs.
<BR>HOWEVER Jaclynn also said:
<BR>
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">We would also consider tissues embedded in glycol methacrylate. We'd like
<BR>to avoid frozen sections because we'd prefer the higher level of detail
<BR>possible with plastic. </FONT><FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0"></BLOCKQUOTE>
<BR></FONT><FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0">
<BR>In THAT case things look better! Would you settle for Sudan black, rather
<BR>than Oil red staining of the lipid? If 'yes' then there is a method - which
<BR>does indeed show even tiny droplets of lipid very clearly. This was worked
<BR>out by the one-time king of GMA staining Peter Gerrits, and can be found in J
<BR>Neurosci Methods, as follows:
<BR>
<BR> Gerrits PO, Brekelmans-Bartels M, Mast L, 's-GrAavenmade EJ, Horobin RW
<BR>and Holstege G. (1992)..
<BR> Staining myelin and myelin-like degradation products in the spinal
<BR>cords of chronic experimental
<BR> allergic encephalomyelitis (Cr-EAE) rats using Sudan Black B staining
<BR>of glycol methacrylate-embedded
<BR> material.
<BR> J. Neuroscience Methods. 45, 99-105
<BR>
<BR>Bye - Richard Horobin
<BR>
<BR>Institute of Biomedical & Life Sciences, University of Glasgow
<BR><B>T direct 01796-474 480 --- E RichardWHorobin@aol.com</B>
<BR><I>"What should we expect? Everything."</I></FONT></HTML>
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