Re: quick question
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Ian, actually I was referring to the stain by Aparicio and Marsden, I need to
search my files for the proper reference.
1% methylene blue in 1% borax
stain for 15-20 seconds on a 70 degree hotplate
rinse in water
stain with 2% basic fuchsin for 15-20 seconds on a hot plate.
rinse in water and coverslip when dry.
i have never tried the polychrome stain you are referring to, i may have to
give it a whirl in the lab.
john
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Ian, actually I was referring to the stain by Aparicio and Marsden, I need to
<BR>search my files for the proper reference.
<BR>
<BR>1% methylene blue in 1% borax
<BR>stain for 15-20 seconds on a 70 degree hotplate
<BR>rinse in water
<BR>stain with 2% basic fuchsin for 15-20 seconds on a hot plate.
<BR>rinse in water and coverslip when dry.
<BR>
<BR>i have never tried the polychrome stain you are referring to, i may have to
<BR>give it a whirl in the lab.
<BR>john</FONT></HTML>
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