Hi,

I have a question about Toluidine Blue. It stains everythign blue as you
said John, But what tissues does it stain pink?
I have 1um thin resin embedded sections

Many Thanks,


Anila

----- Original Message -----
From: J. A. Kiernan <jkiernan@uwo.ca>
To: Lynn Gardner <lynn-gardner@uiowa.edu>
Cc: Histonet <Histonet@pathology.swmed.edu>
Sent: Thursday, May 31, 2001 4:52 AM
Subject: Re: quick question


> On Wed, 30 May 2001, Lynn Gardner wrote:
>
> > Does anyone know if Toludine blue stain done on paraffin or plastic
> > sections will stain collagen?
>
> If you apply toluidine blue (or any other cationic dye) from
> an alkaline solution it will stain everything, including
> collagen fibres. This is useful for thin sections (such as
> 1 um plastic), but less informative for thicker ones.
> ----------------------------------------
> John A. Kiernan
> Department of Anatomy & Cell Biology
> The University of Western Ontario
> London,  Canada   N6A 5C1
>    kiernan@uwo.ca
>    http://publish.uwo.ca/~jkiernan
>
>
>