Re: quick question

From:"J. A. Kiernan" <>

On Wed, 30 May 2001, Lynn Gardner wrote:

> Does anyone know if Toludine blue stain done on paraffin or plastic
> sections will stain collagen?

If you apply toluidine blue (or any other cationic dye) from
an alkaline solution it will stain everything, including
collagen fibres. This is useful for thin sections (such as
1 um plastic), but less informative for thicker ones.
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

<< Previous Message | Next Message >>