Re: Snap freezing.

From:"Dr. Ian Montgomery." <>

<html>  <x-tab>       </x-tab>Use this method myself on occasion, although it's not the best and definitely not for good skeletal muscle histochemistry or EM.<br> <x-tab>        </x-tab>Things to remember: Keep the fluid softly stirring and after the Leidenfrost phenomenon has stopped check that you still have carbon dioxide pellets present keeping the temperature at -80C.<br> Ian.<br>  <br> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail:</font></html>
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