"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
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<x-tab> </x-tab>Use this
method myself on occasion, although it's not the best and definitely not
for good skeletal muscle histochemistry or EM.<br>
<x-tab> </x-tab>Things to
remember: Keep the fluid softly stirring and after the Leidenfrost
phenomenon has stopped check that you still have carbon dioxide pellets
present keeping the temperature at -80C.<br>
Ian.<br>
<br>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>