Re: Pipette Calibration

From:Phyllis Davie <pdavie@phenopath.com>

Here is the table of water weights I inherited from my first supervisor and
have been using ever since.

d.    Table of Volumes and Standard Weights:
Volume of  Deionized Water
(at RT and sea level)            "Standard" Weight
1.55 ml                             1.5455 g
1.00 ml                             0.9971 g
0.75 ml                             0.7478 g
0.50 ml                             0.4985 g
0.40 ml                             0.3988 g
0.30 ml                             0.2991 g
0.20 ml  (200 µl)                   0.1994 g
0.10 ml  (100 µl)                   0.09971 g
0.01 ml  (10 µl)                    0.009971 g
0.005 ml  (5µl)                     0.004985 g

Best of luck.

Phyllis Davie
pdavie@phenopath.com



on 5/31/01 8:58 AM, Nick_Madary@hgsi.com at Nick_Madary@hgsi.com wrote:

> Does anyone know the acceptable range of "weights of water" used when
> calibrating pipettes?  For example the weight of 500 ul should be.....
> I am sure the reference is right in front of me but poor ventilation has
> destroyed many neurons.
> 
> 




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