Re: PBS buffer recipes
|From:||Phyllis Davie <firstname.lastname@example.org>|
Below please find the PBS recipe we have been using for several years.
Best of luck,
Phosphate Buffered Saline (PBS)
Purpose: PBS is general buffer used for washes and dilutions.
1. Sodium chloride, crystals (NaCl)
VWR# EM-SX0420-5 Storage: Room Temperature
2. Sodium phosphate, Dibasic, Anhydrous, (Na2HPO4)
VWR# JT3828-1 Storage: RT
3. Potassium chloride (KCl)
VWR# MK685804 or Sigma # P3911 Storage: RT
4. Potassium phosphate, monobasic, anhydrous (KH2PO4)
Sigma# P5379 or VWR# MK709604 Store: RT
5. Filtered deionized water
1. Add 32 g NaCl
4.6 g Na2HPO4
0.8 g KCl
0.8 g KH2PO4
to filtered deionized water and QS to 4 liters.
The pH should be between 7.28 and 7.60. If the pH does not fall within this
range, remake the PBS solution.
Shelf life: 2 months
Disposal: via sewer
on 5/24/01 9:38 AM, DReynolds@SNBLUSA.com at DReynolds@SNBLUSA.com wrote:
> Hi folks,
> I searched the archives for a pH 7.6 phosphate buffered saline recipe and
> came up empty. Does anyone have one to share? Thanks so much in advance.
> David A. Reynolds, HT(ASCP)
> SNBL USA, Ltd.
> 6605 Merrill Creek Parkway
> Everett, WA 98203
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> Sent: Thursday, May 24, 2001 2:40 AM
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> Subject: non-histology thank you
> Dear all,
> Thank you to those who responded to my posting looking for
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