Dave,
  Below please find the PBS recipe we have been using for several years.

Best of luck,

Phyllis Davie
pdavie@phenopath.com



***************************
Phosphate Buffered Saline (PBS)

Purpose:  PBS is general buffer used for washes and dilutions.

Reagents:
1.  Sodium chloride, crystals (NaCl)
    VWR# EM-SX0420-5    Storage:  Room Temperature
2.  Sodium phosphate, Dibasic, Anhydrous, (Na2HPO4)
    VWR# JT3828-1    Storage:  RT
3.  Potassium chloride (KCl)
    VWR# MK685804  or  Sigma # P3911     Storage:  RT
4.  Potassium phosphate, monobasic, anhydrous (KH2PO4)
    Sigma# P5379  or  VWR# MK709604    Store:  RT
5.  Filtered deionized water


Procedure:
    1.  Add     32 g NaCl
                4.6 g Na2HPO4
                0.8 g KCl
                0.8 g KH2PO4
to filtered deionized water and QS to 4 liters.

The pH should be between 7.28 and 7.60.  If the pH does not fall within this
range, remake the PBS solution.


Storage:    Refrigerated
Shelf life:    2 months
Disposal:    via sewer


*************************



on 5/24/01 9:38 AM, DReynolds@SNBLUSA.com at DReynolds@SNBLUSA.com wrote:

> Hi folks,
> 
> I searched the archives for a pH 7.6 phosphate buffered saline recipe and
> came up empty.  Does anyone have one to share?  Thanks so much in advance.
> 
> Dave
> 
> David A. Reynolds, HT(ASCP)
> SNBL USA, Ltd.
> 6605 Merrill Creek Parkway
> Everett, WA  98203
> 
> 
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> 
> 
> 
> -----Original Message-----
> From: Renton, Lousie, Mrs [mailto:177louie@chiron.wits.ac.za]
> Sent: Thursday, May 24, 2001 2:40 AM
> To: histonet@pathology.swmed.edu
> Subject: non-histology thank you
> 
> 
> Dear all,
> 
> Thank you to those who responded to my posting looking for
> international correspondents. I will forward the information to you all
> individually as soon as I have it.
> 
> best regards
> Louise 
>