Re: PBS buffer recipes

From:Karen Pawlowski <>


I just salinate a 0.1 M phosphate buffer, though I usually don't
make as basic a buffer as pH 7.6.

To do that:

Make stock solutions of monobasic and dibasic phosphate

Monobasic 0.2 M
27.8 gm NaH2PO4-H2O to 1L of distilled H2O

Dibasic 0.2 M
28.38 gm Na2HPO4 (anhydrous) to 1L of distilled H2O

Start with a known quantity of stock Dibasic (say 100 ml) and add 
stock Monobasic until the pH is 7.6, using a stirrer and pH meter.  
Adding more dibasic will raise the pH and adding monobasic will
lower it. Once you've reached pH 7.6, dilute the mixture by half 
(double the volume) to obtain a 0.1M buffer.

Saline is just 0.9% NaCl, so add 0.9 gm per 100 ml of buffer. 

Good Luck,

Karen Pawlowski, Ph.D. wrote:
> Hi folks,
> I searched the archives for a pH 7.6 phosphate buffered saline recipe and
> came up empty.  Does anyone have one to share?  Thanks so much in advance.
> Dave
> David A. Reynolds, HT(ASCP)
> SNBL USA, Ltd.
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