Re: Oil Red O lipid staining

From:Gayle Callis <>

The lipids will be removed by the solvents used in processing.  In tissues
post fixed with osmium tetroxide BEFORE embedding in these plastics, the
osmium will stain lipids black.    

 At 09:42 AM 5/16/01 -0500, you wrote:
>Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
>(Epon or Epon-Araldite)?  If so, has this been done by staining en bloc or
>by staining the sections.  Sections would range from 1-4 microns in
>We would also consider tissues embedded in glycol methacrylate.  We'd like
>to avoid frozen sections because we'd prefer the higher level of detail
>possible with plastic.
>Thank you,
>Jaclynn Lett, Research Assistant
>Faye and Carl Simons Center for Biology of Hearing and Deafness
>Central Institute for the Deaf
>4560 Clayton Ave.
>St. Louis, MO 63110
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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