Re: Oil Red O lipid staining

From:Connie McManus <>


Do you have any fixed, unprocessed tissues to work with??  If so, do
frozens on them instead of your blocks. This is what all the ORO protocols
that I have say to do, which makes sense when you consider that all
processing will subject the tissues to lipid solvents, thus all or most of
the lipids will have been removed and plastics are especially good lipid

Connie McManus  

At 09:42 AM 5/16/01 -0500, Jaclynn Lett wrote:
>Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
>(Epon or Epon-Araldite)?  If so, has this been done by staining en bloc or
>by staining the sections.  Sections would range from 1-4 microns in
>We would also consider tissues embedded in glycol methacrylate.  We'd like
>to avoid frozen sections because we'd prefer the higher level of detail
>possible with plastic.
>Thank you,
>Jaclynn Lett, Research Assistant
>Faye and Carl Simons Center for Biology of Hearing and Deafness
>Central Institute for the Deaf
>4560 Clayton Ave.
>St. Louis, MO 63110
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT

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