Re: Muscle bx's
|From:||Wendy Prime <W.Prime@liverpool.ac.uk>|
This is not an uncommon problem with tissue that has been frozen very
slowly. I suggest you refer to Pearce Histochemistry Volume 1 page 23. I
found it a very interesting read. This explained a phenomenon I had seen
in blocks of pancreatic tissue which had been placed in Nunc tubes and
thrown into, or rather on to, liquid nitrogen. The cells appeared very
shrunken and the connective tissue had huge gaps between the fibres. The
3rd paragraph also made interesting reading. If I understood what the
Pearce had said it implied that ice crystal artefact could be produced by
freezing too rapidly which is contary to the percieved wisdom. i have
since looked at sections of tissue with ice crystal artefact and noticed
that it is often seen around the edge of the block and not in the centre.
Would anyone like to comment?
--On 19 May 2001 07:23 -0400 WHudson436@aol.com wrote:
> I work with muscle bx's and often run into problems with specimen that
> arrive frozen. When they are still frozen and are packed in dry ice.
> One of the most frustrating problems that occur with some of the
> specimens is after I place the frozen specimen in the cryostat set at-20
> the muscle becomes soft and "squishy." I have placed them directly into
> the cryostat still frozen as well as letting them come to room
> temperature and then snap freezing the sample. They still seem to get
> equally soft after they have been in the cryostat. The muscle acts as
> if something has caused it to require a lower temperature to keep it
> frozen solid. I do not run into that problem with the muscle bx's that
> arrive fresh and that I snap freeze.
> Can any one suggest good reference books or web sites for established
> technique on muscle bx's. I would be very appreciated of any and all
> hints from techs who work with muscle bx's.
> Thank you so much.
> N. Hudson
University of Liverpool
0151 706 4503
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