Re: Fwd: Cryostat phenomenon
|From:||Ej Hurley <firstname.lastname@example.org>|
I have encountered that same problem from time to time. It usually happens when my room ambient temperature is warmer than usual. I have found if I spray the back of the slide with a quick burst of cytocool it allieviates the problem. I hope this helps.
San Bernardino, CA
>>> "Dr. Ian Montgomery." <email@example.com> 05/17/01 05:03AM >>>
Been there many times over the years. Tried earthing the
microtome, whole cabinet, zapping the inside of the chamber with an
antistatic pistol but with little success. The thing I found works, to a
degree, is the Basil Fawlty. Jump up and down on the spot, curse and swear
and warn the block that if it doesn't behave you'll give it a damn good
thrashing. Failing that I just go and stare at My Little Calender Girl 2001
calender. Doesn't help the block but gets me into a more relaxed tranquil
state for the next effort.
>Date: Thu, 17 May 2001 08:54:05 +0100
>From: "firstname.lastname@example.org" <T.Hacker@har.mrc.ac.uk>
>Subject: Cryostat phenomenon
>To: "'Histonet'" <Histonet@pathology.swmed.edu>
> Has anyone encountered a phenomenon that I occasionally get
>with cryostat frozen sectioning. I assume it is due to static
>electrical charges? the sections tend to "leap" from the knife edge
>onto the slide, the slide may be some several centimeters from the
>There is no logic or consistency to this, irrespective of tissue,
>fixation, temperature, type of slide; but if it is going to happen then
>you can be sure it is when cutting a tiny piece of tissue where
>every section is required! It will often disappear as suddenly as it
>started, sometimes quite impressive when a section fly's several
>cms. to the slide and occasionally lands flat and crease free!
>Any idea's?Oh, and I don't wear nylons (not in work anyway).
>Medical Research Council,
>Oxfordshire, OX11 ORD
>01235 834393 x360
Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Tel: 0141 339 8855. Extn:6602.
Fax: 0141 330 2923
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