Re: Fwd: Cryostat phenomenon

From:Becky Dilallo <>

<P>Terry,</P> <P>I have found that sometimes the embedding matrix can effect how impossible the static will be.  You could always try switching brands and see what happens.</P> <P>good luck,</P> <P>Becky DiLallo</P> <P>Pittsburgh, PA</P> <P> </P> <P><BR> </P> <P>  <BR> <P>  <B><I>"Dr. Ian Montgomery." <></I></B> wrote: <BR> <BLOCKQUOTE style="BORDER-LEFT: #1010ff solid 2px; MARGIN-LEFT: 5px; PADDING-LEFT: 5px"><HTML>Terry,<BR><X-TAB>        </X-TAB>Been there many times over the years. Tried earthing the microtome, whole cabinet, zapping the inside of the chamber with an antistatic pistol but with little success. The thing I found works, to a degree, is the Basil Fawlty. Jump up and down on the spot, curse and swear and warn the block that if it doesn't behave you'll give it a damn good thrashing. Failing that I just go and stare at My Little Calender Girl 2001 calender. Doesn't help the block but gets me into a more relaxed tranquil state for the next effort.<BR>Ian.<BR><BR><BR><BR><BR> <BLOCKQUOTE class=cite type = cite cite>Date: Thu, 17 May 2001 08:54:05 +0100<BR>From: "" <><BR>Subject: Cryostat phenomenon<BR>To: "'Histonet'" <><BR><BR> Has anyone encountered a phenomenon that I occasionally get <BR>with cryostat frozen sectioning. I assume it is due to static <BR>electrical charges? the sections tend to "leap" from the knife edge <BR>onto the slide, the slide may be some several centimeters from the <BR>section/knife holder.<BR>There is no logic or consistency to this, irrespective of tissue, <BR>fixation, temperature, type of slide; but if it is going to happen then <BR>you can be sure it is when cutting a tiny piece of tissue where <BR>every section is required! It will often disappear as suddenly as it <BR>started, sometimes quite impressive when a section fly's several <BR>cms. to the slide and occasionally lands flat and crease free! <BR>Any idea's?Oh, and I don't <P></X-SIGSEP><FONT color=#0000ff>Dr. Ian Montgomery,<BR>West Medical Building,<BR>University of Glasgow,<BR>Glasgow,<BR>G12 8QQ.<BR>Tel: 0141 339 8855.  Extn:6602.<BR>Fax: 0141 330 2923<BR>e-mail:</FONT> </P></BLOCKQUOTE><p><br><hr size=3D1><b>Do You Yahoo!?</b><br> <a href="">Yahoo! Auctions $2 Million Sweepstakes </a> - Got something to sell?
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