Hi Susan
Although we are doing our IHC mostly on human tissue, we occasionally help
out our colleagues in vet path. We found clone MNF116 (available from
various vendors, e.g. Dako M0821) to work very nicely in dogs. MNF116 is a
'broad-spectrum' anti-CK mAb that recognizes CKs 5, 6, 8, 17 and possibly
19. CKs 5 and 17 are basic CKs of squamous, CK 8 is typical for non-squamous
epithelia. Alternatively you can try Dako's polyclonal rb-a-Keratin, which
is another broad-spectrum Ab reactive in dogs. Good luck!
Best regards

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

---Original message---
From: Susan Q Wells <susan.wells@bms.com>
To: <histonet@pathology.swmed.edu>
Sent: 29. May 2001 20:26
Re: Cytokeratin staining in canines


> Hi fellow histonetters!
> Anyone out there doing a wide spectrum cytokeratin stain on FFPE
> dog tissue?
> I'd appreciate input on reagents,vendors,etc.
> Thanks -
>
> Susan Wells, HT
> Assoc.Research Sci.
> Bristol Myers Sqibb
>
>