Re: Celloidin Technique
|From:||Shirley Powell <powell_sa@Mercer.EDU>|
I prefer the term antiques, but thanks, if I did not have something to stick to,
I would fall apart. :)
Mary Bryhan wrote:
> I too remember celloidinization as part of my everyday life, back in the
> day. Don't feel bad Shirley... us dinosaurs have to stick together.
> Happy Memorial Day to all !
> Mary Bryhan
> Petoskey, MI
> ----- Original Message -----
> From: Shirley Powell <powell_sa@Mercer.EDU>
> To: Greenfield, Barbara <email@example.com>
> Cc: Histonet <Histonet@pathology.swmed.edu>
> Sent: Thursday, May 24, 2001 9:11 PM
> Subject: Re: Celloidin Technique
> > At the risk of telling you how many years ago I started in this field, I
> > remember that the section, after deparaffinization step, from a 95%
> alcohol was
> > placed for 5 minutes in 1% Celloidin in equal parts of alcohol and ether,
> > eeegads. It then was placed in 70% alcohol for 10 minutes to harden. The
> > stain was performed and the celloidin removed in the dehydration step in
> > parts of alcohol and ether before clearing and coverslipping. I found
> this in
> > Culling's 3rd Edition of Handbook of Histopathological and Histochemical
> > Techniques, page 142 under Celloidinization. It worked for me way back
> > Shirley Powell
> > "Greenfield, Barbara" wrote:
> > > A question for the gurus of Histology.
> > >
> > > Many years ago (and I emphasize many) the lab I trained in used a
> > > for post treating paraffin sections to keep them on the slide through
> > > of the silver staining techniques. It involved dipping the mounted,
> > > deparaffinized slides in a mixture containing celloidin. Does this ring
> > > bell out there, and if so do you remember the concoction and procedure
> > > details?
> > >
> > > Any pearls of wisdom would be appreciated.
> > >
> > > Barbara Greenfield
> > > Laboratory Operations Manager
> > > Erie Scientific, Co.
> > > 20 Post Road
> > > Portsmouth N.H. 03801
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