Re: Celloidin Technique
|From:||Mary Bryhan <firstname.lastname@example.org>|
I too remember celloidinization as part of my everyday life, back in the
day. Don't feel bad Shirley... us dinosaurs have to stick together.
Happy Memorial Day to all !
----- Original Message -----
From: Shirley Powell <powell_sa@Mercer.EDU>
To: Greenfield, Barbara <email@example.com>
Cc: Histonet <Histonet@pathology.swmed.edu>
Sent: Thursday, May 24, 2001 9:11 PM
Subject: Re: Celloidin Technique
> At the risk of telling you how many years ago I started in this field, I
> remember that the section, after deparaffinization step, from a 95%
> placed for 5 minutes in 1% Celloidin in equal parts of alcohol and ether,
> eeegads. It then was placed in 70% alcohol for 10 minutes to harden. The
> stain was performed and the celloidin removed in the dehydration step in
> parts of alcohol and ether before clearing and coverslipping. I found
> Culling's 3rd Edition of Handbook of Histopathological and Histochemical
> Techniques, page 142 under Celloidinization. It worked for me way back
> Shirley Powell
> "Greenfield, Barbara" wrote:
> > A question for the gurus of Histology.
> > Many years ago (and I emphasize many) the lab I trained in used a
> > for post treating paraffin sections to keep them on the slide through
> > of the silver staining techniques. It involved dipping the mounted,
> > deparaffinized slides in a mixture containing celloidin. Does this ring
> > bell out there, and if so do you remember the concoction and procedure
> > details?
> > Any pearls of wisdom would be appreciated.
> > Barbara Greenfield
> > Laboratory Operations Manager
> > Erie Scientific, Co.
> > 20 Post Road
> > Portsmouth N.H. 03801
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