I too remember celloidinization as part of my everyday life, back in the
day.  Don't feel bad Shirley... us dinosaurs have to stick together.

Happy Memorial Day to all !

Mary Bryhan
Petoskey, MI


----- Original Message -----
From: Shirley Powell <powell_sa@Mercer.EDU>
To: Greenfield, Barbara <bgreenfield@eriesci.com>
Cc: Histonet <Histonet@pathology.swmed.edu>
Sent: Thursday, May 24, 2001 9:11 PM
Subject: Re: Celloidin Technique


> At the risk of telling you how many years ago I started in this field, I
> remember that the section, after deparaffinization step, from a 95%
alcohol was
> placed for 5 minutes in 1% Celloidin in equal parts of alcohol and ether,
> eeegads.  It then was placed in 70% alcohol for 10 minutes to harden.  The
> stain was performed and the celloidin removed in the dehydration step in
equal
> parts of alcohol and ether before clearing and coverslipping.  I found
this in
> Culling's 3rd Edition of Handbook of Histopathological and Histochemical
> Techniques, page 142 under Celloidinization.  It worked for me way back
then.
>
> Shirley Powell
>
>
> "Greenfield, Barbara" wrote:
>
> > A question for the gurus of Histology.
> >
> > Many years ago (and I emphasize many) the lab I trained in used a
technique
> > for post treating paraffin sections to keep them on the slide through
some
> > of the silver staining techniques.  It involved dipping the mounted,
> > deparaffinized slides in a mixture containing celloidin.  Does this ring
a
> > bell out there, and if so do you remember the concoction and procedure
> > details?
> >
> > Any pearls of wisdom would be appreciated.
> >
> > Barbara Greenfield
> > Laboratory Operations Manager
> > Erie Scientific, Co.
> > 20 Post Road
> > Portsmouth N.H. 03801
>
>
>