Re: Celloidin Technique

From:Bill Sinai <>

You are so right about this being a technique used many years ago.
We made a 5% solution of celloidin (Merck cellusolve) in a 50/50
ether/ethanol 100% mix.  It took forever to dissolve and then it had to be
stored at 4degrees C.  This was a stock solution and we diluted it to 1% in
ethanol 100%.  This solution was substituted for the last ethanol 100%
rehydrating alcohol and then slides placed into 70% alcohol to harden the

We have not used this technique for close to twenty years due to the
cleanliness of modern day slides and the adhesives available.

Subject: Celloidin Technique

> A question for the gurus of Histology.
> Many years ago (and I emphasize many) the lab I trained in used a
> for post treating paraffin sections to keep them on the slide through some
> of the silver staining techniques.  It involved dipping the mounted,
> deparaffinized slides in a mixture containing celloidin.  Does this ring a
> bell out there, and if so do you remember the concoction and procedure
> details?
> Any pearls of wisdom would be appreciated.
> Barbara Greenfield
> Laboratory Operations Manager
> Erie Scientific, Co.
> 20 Post Road
> Portsmouth N.H. 03801

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