Re: Celloidin Technique
From: | Bill Sinai <bills@icpmr.wsahs.nsw.gov.au> |
You are so right about this being a technique used many years ago.
We made a 5% solution of celloidin (Merck cellusolve) in a 50/50
ether/ethanol 100% mix. It took forever to dissolve and then it had to be
stored at 4degrees C. This was a stock solution and we diluted it to 1% in
ethanol 100%. This solution was substituted for the last ethanol 100%
rehydrating alcohol and then slides placed into 70% alcohol to harden the
celloidin.
We have not used this technique for close to twenty years due to the
cleanliness of modern day slides and the adhesives available.
************************************************
Subject: Celloidin Technique
> A question for the gurus of Histology.
>
> Many years ago (and I emphasize many) the lab I trained in used a
technique
> for post treating paraffin sections to keep them on the slide through some
> of the silver staining techniques. It involved dipping the mounted,
> deparaffinized slides in a mixture containing celloidin. Does this ring a
> bell out there, and if so do you remember the concoction and procedure
> details?
>
> Any pearls of wisdom would be appreciated.
>
> Barbara Greenfield
> Laboratory Operations Manager
> Erie Scientific, Co.
> 20 Post Road
> Portsmouth N.H. 03801
>
>
<< Previous Message | Next Message >>