RE: mouse perfusion
This is particularly addressed to John K., who tells us:
"You should not move a piece of tissue from a phosphate-buffered formaldehyde
fixative into a zinc-formalin, because the zinc ions will make
insoluble zinc phosphate as soon as they bump into phosphate ions
derived from the perfused liquid."
For those of us that love zinc fixation, but don't have it avalilable other than as a post-fixative for a few hours post cut-up when it is our turn on surgicals, what are us poor boys to do?
I know the problem and try to minimise it with a wash beforehand, but realise that this is less than satisfactory.
What is the way to go in these circumstances?
Is an alcoholic zinc a better option?
If so, what (non-commercial) formulation?
Terry L Marshall
Rotherham General Hospital, Yorkshire
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