RE: endogenous peroxidase
|From:||"Plummer, Timothy B." <Plummer.Timothy@mayo.edu>|
The following treatment is used after a PBS rinse and is followed by PBS
rinses prior to the application of a blocking serum or primary Ab step.
immerse slides in fresh 0.3% H2O2 prepared in 0.1% Sodium Azide(aq) for
prepare fresh and place in coplin jar
3% H2O2 5ml
0.1% NaAzide 45 ml
Dispose of Na azide solution in accordance with local and other regulations.
Use of azide and hydrogen peroxide as an inhibitor for endogenous
in the immunoperoxidase method.
J HISTOCHEM CYTOCHEM 35:1457-1460,
This works very well,
> -----Original Message-----
> From: email@example.com [SMTP:T.Hacker@har.mrc.ac.uk]
> Sent: Thursday, May 31, 2001 10:31 AM
> To: 'Histonet'
> Subject: endogenous peroxidase
> What are you using for blocking endogenous peroxidase in acetone
> fixed frozen sections?
> I have used a weak solution of H202 which is not always effective,
> too strong and I get excessive "bubbling" of the tissue. Yes, I
> know, try a strength in between, but I was wondering if there is
> something more appropriate and less aggressive.
> I look forward to a wealth of knowledge and experience, preferably
> by 9am GMT tomorrow.
> Many thanks,
> Terry Hacker,r
> Medical Research Council,
> Oxfordshire, OX11 ORD
> 01235 834393 x360
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