RE: automated IHC

From:"Morken, Tim" <tim9@cdc.gov>

I've been doing immunos in a clinical setting for almost 20 years. I started
out doing batches of 40 slides a week and have done up to 200 slides a day.
I sincerely believe that if you are doing any number of immunos on a regular
basis that automation will be worthwhile. It  is more consistent, less prone
to mistakes and allows you do other things without interuption during the
run. Just ask yourself, why pay someone to drain and wipe slides, and apply
reagents  when a machine can do it? that person is more valuable doing
something else.

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Jerry Chi-Yuan Hu [mailto:ragnarok@ruf.rice.edu]
Sent: Tuesday, May 29, 2001 2:07 PM
To: ragnarok@rice.edu
Cc: histonet@pathology.swmed.edu
Subject: automated IHC



Hello Everyone,

One of our collaborators doing IHC has ranted and raved about how much
easier his job would be with an automated IHC stainer.  We will also be
doing IHC in our lab, and I would like to know at which point such a
machine would be helpful (for instance, if somebody does 10 slides a day
vs somebody who does 90 slides a day).  I welcome any vendors to contact
me directly so that I can better understand which steps in the IHC
staining process will be automated in each model.  If somebody has a
favorite model I would also be interested in hearing why.  Any comments
will be greatly appreciated.

**********
Jerry Hu
Musculoskeletal Bioengineering Laboratory
Rice University
Department of Bioengineering - MS 142
P.O. Box 1892, Houston, Texas 77251-1892
Tel: (713) 348-6393
Fax: (713) 348-5877
email: ragnarok@rice.edu
**********




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