RE: Oil Red O lipid staining

The problem with trying to do lipid staining in epon or plastic embedded
tissue is that inorder to embed in epon or gma you usually have to process
in solvents which will dissolve the lipids not to mention that the plastic
itself is a solvent, at least GMA is somewhat.
Patsy Ruegg

		-----Original Message-----
		From:	Jaclynn Lett []
		Sent:	Wednesday, May 16, 2001 8:43 AM
		To:	HistoNet (E-mail)
		Subject:	Oil Red O lipid staining

		Has anyone used Oil Red O to stain lipids in tissues
embedded in plastics
		(Epon or Epon-Araldite)?  If so, has this been done by
staining en bloc or
		by staining the sections.  Sections would range from 1-4
microns in

		We would also consider tissues embedded in glycol
methacrylate.  We'd like
		to avoid frozen sections because we'd prefer the higher
level of detail
		possible with plastic.

		Thank you,

		Jaclynn Lett, Research Assistant

		Faye and Carl Simons Center for Biology of Hearing and
		Central Institute for the Deaf
		4560 Clayton Ave.
		St. Louis, MO 63110

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