|From:||Philip Oshel <email@example.com>|
I know of no adequate substitute for glutaraldehyde as a fixtive for
EM. I do know of substitutes that are much *more* hazardous, but none
that are less troublesome.
For many high-resolution TEM and SEM procedures of cultured cells
(therefore probably not applicable in clinical settings), glut can be
used without formalin. I wouldn't want to try the vice-versa, though.
I don't see why the inspection agency or your pathologist would get
their knickers in a knot over glut. Any self-respecting histology or
microscopy facility has much worse chemicals on its shelves.
>> From: Nocito, Joseph
>> Sent: Friday, May 25, 2001 6:33 AM
>> To: 'Histonet'
>> Subject: Gluteraldehyde
>> We are currently going through a JCAHO inspection (what a year, CAP, CLIA
>> and now JCAHO, any one else out there?) Any way, the inspectors went
>> the roof when they saw that we have gluteraldehyde. Now, the hospital
>> Safety board is in an up roar. Why is gluteraldehyde such a dirty word.
>> know the general hazards, but is there something new that I might have
>> missed? Also, my pathologist wants to know if there is a substitute
>> fixative for electron microscopy. Once again, I thank you for your help.
>> Joe Nocito, B.S., HT(ASCP)QIHC
>> Histology Supervisor
>> Christus Santa Rosa Hospitals
>> San Antonio, Texas
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
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