RE: FAM fixative and immunohistochemistry

From:"Greer, Patricia" <pwg1@cdc.gov>

We have used FAM for fixing cytospins or smears (60-90 seconds) prior to
immunohistochemistry.  However our FAM recipe is formaldehyde, 40% (1ml),
acetone (19ml) and methanol (19ml).  It worked fine for the specimens we
had, it gave good morphological detail of the cells and good immunostaining;
but, I would be concerned about adequate fixation for pieces of tissue.

Pat Greer
CDC
Infectious Disease Pathology 





-----Original Message-----
From: DReynolds@SNBLUSA.com [mailto:DReynolds@SNBLUSA.com]
Sent: Wednesday, May 30, 2001 5:30 PM
To: histonet@pathology.swmed.edu
Subject: FAM fixative and immunohistochemistry


Hi all,

I have a question for all of the chemistry and IHC geniuses out there.  I
have a request that specifies formaldehyde-acetic acid-methanol (FAM)
fixation on brain tissue, which after routine processing and paraffin
embedding requires immunohistochemistry.  Early IHC testing on this tissue
has been less than satisfactory.  Does this fixative pose a problem?  Would
10% neutral-buffered formalin be more appropriate?  (Okay, that's two
questions..sorry)......

Thanks

Dave

David A. Reynolds, HT(ASCP)
SNBL USA, Ltd.
6605 Merrill Creek Parkway
Everett, WA  98203


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