RE: FAM fixative and immunohistochemistry

From:"Greer, Patricia" <>

We have used FAM for fixing cytospins or smears (60-90 seconds) prior to
immunohistochemistry.  However our FAM recipe is formaldehyde, 40% (1ml),
acetone (19ml) and methanol (19ml).  It worked fine for the specimens we
had, it gave good morphological detail of the cells and good immunostaining;
but, I would be concerned about adequate fixation for pieces of tissue.

Pat Greer
Infectious Disease Pathology 

-----Original Message-----
From: []
Sent: Wednesday, May 30, 2001 5:30 PM
Subject: FAM fixative and immunohistochemistry

Hi all,

I have a question for all of the chemistry and IHC geniuses out there.  I
have a request that specifies formaldehyde-acetic acid-methanol (FAM)
fixation on brain tissue, which after routine processing and paraffin
embedding requires immunohistochemistry.  Early IHC testing on this tissue
has been less than satisfactory.  Does this fixative pose a problem?  Would
10% neutral-buffered formalin be more appropriate?  (Okay, that's two



David A. Reynolds, HT(ASCP)
6605 Merrill Creek Parkway
Everett, WA  98203

Confidentiality Notice: This email, its contents and attachments are
confidential and may contain privileged information. It is intended solely
for the use of addressee(s) only. Any use, copying or disclosure of this
communication or attachments to any other person is expressly prohibited
without written permission of SNBL-USA,Ltd. If you receive this message in
error, please notify the sender at SNBL USA, Ltd. immediately by return
e-mail, telephone +1 425 407 0121, or fax +1 425 407 8601. We appreciate
your cooperation.

<< Previous Message | Next Message >>