Cd4 should work fine with hrp, I do rat anti-mouse cd4 on frozen mouse
tissue using an abc/hrp kit from Santa Cruz it works fine.
Patsy Ruegg

		-----Original Message-----
		From:	Terrett, Barb [mailto:Barb.Terrett@uhn.on.ca]
		Sent:	Tuesday, May 22, 2001 2:04 PM
		To:	'histonet@pathology.swmed.edu'
		Subject:	C4d on frozen renal Bx's

		Hi all,
		Is anyone out there having any success staining frozen (or
paraffin) renal
		biopsies with C4d using a peroxidase method rather than IF?
I was told that
		it should work as a peroxidase method, and as I understand
it the vessels
		should be coming up. 
		Thanks for any help you can give.
		b