Oil Red O lipid staining

From:Jaclynn Lett <jlett@cid.wustl.edu>

Has anyone used Oil Red O to stain lipids in tissues embedded in plastics
(Epon or Epon-Araldite)?  If so, has this been done by staining en bloc or
by staining the sections.  Sections would range from 1-4 microns in
thickness.

We would also consider tissues embedded in glycol methacrylate.  We'd like
to avoid frozen sections because we'd prefer the higher level of detail
possible with plastic.

Thank you,

Jaclynn Lett, Research Assistant     jlett@cid.wustl.edu

Faye and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110



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