Help with Tape Transfer System
From: | Linda Jenkins <jlinda@ces.clemson.edu> |
Dear Histonetters,
We are currently attempting to use the Instrumedics paraffin tape
transfer system to capture hard-to-cut paraffin sections. I am using
Surgipath embedding paraffin and the A/O 820 microtome with the disposable
blade holder (nonadjustable). The problem is - the tape does not want to
adhere to the faced block. I have tried varying the speed of sectioning,
chilling the tape, and varying the thickness from 3 - 10 microns. If I'm
lucky (not usually) I can get a partial piece of tissue to adhere to the
tape. I also tried this on a blank block with the same results. Also, on
the partial pieces I manage to obtain, there is a heavy eosin like
background staining. Why bother? Well...on those partial pieces that I
manage to obtain - the structure is incredible - I want the whole section
very badly. Anyone else using this system that would care to share their
expertise?
Thanks,
Linda
*********************************
Linda Jenkins, HT
Clemson University
Department of Bioengineering
864.656.5553
**********************************
<< Previous Message | Next Message >>