Fwd: Re: Oil Red O staining.

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> <x-tab>        </x-tab>The block was post fixed with osmium tetroxide/cacodylate then stained with Sudan black. It was only a wee trial just to see what happened. At the time I naively thought that the Sudan black must be binding to the osmium fixed fat. It was only a once of on a single slide. I stained another slide with Toluidine blue/Pyronine Y and it was just as lovely. Goodness knows what the answer was. At the time I used to try all sorts of staining combinations on semi-thin Glut/Osmium resin sections. Some were awful but others showed promise, unfortunately time didn't permit further investigation. <br> <x-tab>        </x-tab>The best staining for Glut/Osmium resin sections, in our hands was Toluidine Blue/Pyronine Y (Ito & Winchester 1963 J. Cell Biol) and a Polychrome technique (Pasyk, Bartok & Fabry 1989 Stain Technol <u>64</u> (3) 149.<br> Ian.   <br><br> <br> <blockquote type=cite class=cite cite>X-Sender: uvsgc@trex2.oscs.montana.edu<br> X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)<br> Date: Thu, 17 May 2001 09:01:55 -0600<br> To: "Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk><br> From: Gayle Callis <uvsgc@montana.edu><br> Subject: Re: Oil Red O staining.<br><br> Question:  Was the lipid staining the result of post fixation with osmium<br> or from Sudan Black?  Or did you try one block not post fixed with osmium,<br> stained with sudan black and still have same results?  Just curious.<br><br> Nice protocol!!<br><br> <br>  At 01:16 PM 5/17/01 +0100, you wrote:<br> >         Years ago I prepared a demonstration slide of testes for the<br> >Leydig cells that came from a conventional EM block, Glut/Cacod and Osmium.<br> >1 micron section stained with Sudan black then Toluidine blue. Beautiful<br> >result but it was only a one-off, although something I'll try again if<br> needed. <br> > Ian. <br> >  Dr. Ian Montgomery,<br> > West Medical Building,<br> > University of Glasgow,<br> > Glasgow,<br> > G12 8QQ.<br> >  Extn:6602.<br> > Fax: 0141 330 2923<br> > e-mail: ian.montgomery@bio.gla.ac.uk <br> Gayle Callis<br> MT,HT,HTL(ASCP)<br> Histopathology Supervisor<br> Veterinary Molecular Biology<br> Montana State University - Bozeman<br> Bozeman MT 59717-3610<br><br> 406 994-6367<br> 404 994-4303 (FAX)</blockquote> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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