"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
<x-tab> </x-tab>The block
was post fixed with osmium tetroxide/cacodylate then stained with Sudan
black. It was only a wee trial just to see what happened. At the time I
naively thought that the Sudan black must be binding to the osmium fixed
fat. It was only a once of on a single slide. I stained another slide
with Toluidine blue/Pyronine Y and it was just as lovely. Goodness knows
what the answer was. At the time I used to try all sorts of staining
combinations on semi-thin Glut/Osmium resin sections. Some were awful but
others showed promise, unfortunately time didn't permit further
investigation. <br>
<x-tab> </x-tab>The best
staining for Glut/Osmium resin sections, in our hands was Toluidine
Blue/Pyronine Y (Ito & Winchester 1963 J. Cell Biol) and a Polychrome
technique (Pasyk, Bartok & Fabry 1989 Stain Technol <u>64</u> (3)
149.<br>
Ian. <br><br>
<br>
<blockquote type=cite class=cite cite>X-Sender:
uvsgc@trex2.oscs.montana.edu<br>
X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)<br>
Date: Thu, 17 May 2001 09:01:55 -0600<br>
To: "Dr. Ian Montgomery."
<ian.montgomery@bio.gla.ac.uk><br>
From: Gayle Callis <uvsgc@montana.edu><br>
Subject: Re: Oil Red O staining.<br><br>
Question: Was the lipid staining the result of post fixation with
osmium<br>
or from Sudan Black? Or did you try one block not post fixed with
osmium,<br>
stained with sudan black and still have same results? Just
curious.<br><br>
Nice protocol!!<br><br>
<br>
At 01:16 PM 5/17/01 +0100, you wrote:<br>
> Years ago I prepared
a demonstration slide of testes for the<br>
>Leydig cells that came from a conventional EM block, Glut/Cacod and
Osmium.<br>
>1 micron section stained with Sudan black then Toluidine blue.
Beautiful<br>
>result but it was only a one-off, although something I'll try again
if<br>
needed. <br>
> Ian. <br>
> Dr. Ian Montgomery,<br>
> West Medical Building,<br>
> University of Glasgow,<br>
> Glasgow,<br>
> G12 8QQ.<br>
> Extn:6602.<br>
> Fax: 0141 330 2923<br>
> e-mail: ian.montgomery@bio.gla.ac.uk <br>
Gayle Callis<br>
MT,HT,HTL(ASCP)<br>
Histopathology Supervisor<br>
Veterinary Molecular Biology<br>
Montana State University - Bozeman<br>
Bozeman MT 59717-3610<br><br>
406 994-6367<br>
404 994-4303 (FAX)</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>