Fwd: Cryostat phenomenon

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> Terry,<br> <x-tab>        </x-tab>Been there many times over the years. Tried earthing the microtome, whole cabinet, zapping the inside of the chamber with an antistatic pistol but with little success. The thing I found works, to a degree, is the Basil Fawlty. Jump up and down on the spot, curse and swear and warn the block that if it doesn't behave you'll give it a damn good thrashing. Failing that I just go and stare at My Little Calender Girl 2001 calender. Doesn't help the block but gets me into a more relaxed tranquil state for the next effort.<br> Ian.<br><br> <br><br> <blockquote type=cite class=cite cite>Date: Thu, 17 May 2001 08:54:05 +0100<br> From: "t.hacker@har.mrc.ac.uk" <T.Hacker@har.mrc.ac.uk><br> Subject: Cryostat phenomenon<br> To: "'Histonet'" <Histonet@pathology.swmed.edu><br><br>  Has anyone encountered a phenomenon that I occasionally get <br> with cryostat frozen sectioning. I assume it is due to static <br> electrical charges? the sections tend to "leap" from the knife edge <br> onto the slide, the slide may be some several centimeters from the <br> section/knife holder.<br> There is no logic or consistency to this, irrespective of tissue, <br> fixation, temperature, type of slide; but if it is going to happen then <br> you can be sure it is when cutting a tiny piece of tissue where <br> every section is required! It will often disappear as suddenly as it <br> started, sometimes quite impressive when a section fly's several <br> cms. to the slide and occasionally lands flat and crease free! <br> Any idea's?Oh, and I don't wear nylons (not in work anyway).<br> Terry.<br> Terry Hacker,<br> Medical Research Council,<br> Harwell,<br> Didcot,<br> Oxfordshire, OX11 ORD<br> 01235 834393 x360</blockquote> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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