Has anyone encountered a phenomenon that I occasionally get 
with cryostat frozen sectioning. I assume it is due to static 
electrical charges? the sections tend to "leap" from the knife edge 
onto the slide, the slide may be some several centimeters from the 
section/knife holder.
There is no logic or consistency to this, irrespective of tissue, 
fixation, temperature, type of slide; but if it is going to happen then 
you can be sure it is when cutting a tiny piece of tissue where 
every section is required! It will often disappear as suddenly as it 
started, sometimes quite impressive when a section fly's several 
cms. to the slide and occasionally lands flat and crease free! 
Any idea's?Oh, and I don't wear nylons (not in work anyway).
Terry.
Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360