Hello All,

I need info/help/comments in regard to IHC staining for Cox-2 in 
rats, mice, canine, and human FFPE blocks. Is Ag retreival 
routinely used? One protocol I have, says they permeablized the 
section with TNB-BB (I don't know what that stands for, but I do 
have a recipe for it and it contains triton-X-100). What's with 
permeablizing for IHC? Has anyone found TSA (tyramide) to be 
absolutely necessary?? Thanks in advance for any and all info 
offered.
Cheers! 
Greg