Brittle blocks

From:Barry Rittman <>

would you please let us know.
1.    Temperature at which you are operating your cryostat.
2.    The tissue you are cutting
3.    Approximate size of the blocks
4.    Thickness of sections.


I've just subscribed to Histonet and so far it seems great.  Now I was
just wondering if anyone could give me some advice.

I snap freeze samples in OCT compound in isopentane cooled with liquid
nitrogen.  Unfortunately quite a few of the blocks I get are very
brittle and very difficult to get slices from, the slices just flake
into very small strips which I can't use.  So, is there anyway I can
improve a block when its this brittle, so that I can make sections from


Phil Bergin

Department of Medical Microbiology and Immunology
G#246#teborg University
Box 435
405 30 G#246#teborg

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