Brittle blocks: more info.

From:Phil Bergin <>


Thanks for the advice so far, I've been asked for some more information
so here it is:

Our cryostat is at -20 C and I tend to leave my blocks for at least 30
min before I start to cut them.

The samples are gastric biopsies from the stomach antrum and duodenum.

The blocks are frozen in cryomoulds 10mm x 10 mm x 5mm in size.

I cut sections at 8um in thickness.

After I freeze the blocks I store them in liquid nitrogen for transport
to the lab, where I keep them in a -80 freezer till I use them.

Thanks again for the advice,


Department of Medical Microbiology and Immunology
Göteborg University
Box 435
405 30 Göteborg

Phone: +46 (0)31-342 4727
Fax:   +46 (0)31-82 01 60

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