Brittle blocks

From:Phil Bergin <>


I've just subscribed to Histonet and so far it seems great.  Now I was
just wondering if anyone could give me some advice.

I snap freeze samples in OCT compound in isopentane cooled with liquid
nitrogen.  Unfortunately quite a few of the blocks I get are very
brittle and very difficult to get slices from, the slices just flake
into very small strips which I can't use.  So, is there anyway I can
improve a block when its this brittle, so that I can make sections from


Phil Bergin

Department of Medical Microbiology and Immunology
Göteborg University
Box 435
405 30 Göteborg

Phone: +46 (0)31-342 4727
Fax:   +46 (0)31-82 01 60

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