Brittle blocks
From: | Phil Bergin <philip.bergin@microbio.gu.se> |
Hi,
I've just subscribed to Histonet and so far it seems great. Now I was
just wondering if anyone could give me some advice.
I snap freeze samples in OCT compound in isopentane cooled with liquid
nitrogen. Unfortunately quite a few of the blocks I get are very
brittle and very difficult to get slices from, the slices just flake
into very small strips which I can't use. So, is there anyway I can
improve a block when its this brittle, so that I can make sections from
it?
Thanks,
Phil Bergin
Department of Medical Microbiology and Immunology
Göteborg University
Box 435
405 30 Göteborg
Sweden
Phone: +46 (0)31-342 4727
Fax: +46 (0)31-82 01 60
E-mail: philip.bergin@microbio.gu.se
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