"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
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<x-tab> </x-tab>I have a
client who is going to Trinidad for 4 weeks collecting Xenopus larvae
then bringing them back to Glasgow for whole mount staining. We will be
looking for Tyrosine Hydroxylase in hatching glands after fixation in 2%
trichloroacetic acid/PBS. 2% TCA is the fix of choice, others have been
tried but in this instance TCA is the preferred method. <br>
What I'm not sure about is handling after fixation. Do I leave in TCA for
the 4 weeks or place in PBS until they come to Glasgow. This is a field
trip so further processing is not possible, it has to be fix or fix then
buffer before the flight back to Scotland.<br>
<x-tab> </x-tab>All this
because my microscopy brief was expanded. Wish it was the wallet and not
just the waist line that is expanding.<br>
Ian. <br>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>