From:"Dr. Ian Montgomery." <>

<html> <x-tab>        </x-tab>I have a client who is going to Trinidad for 4 weeks collecting Xenopus larvae then bringing them back to Glasgow for whole mount staining. We will be looking for Tyrosine Hydroxylase in hatching glands after fixation in 2% trichloroacetic acid/PBS. 2% TCA is the fix of choice, others have been tried but in this instance TCA is the preferred method. <br> What I'm not sure about is handling after fixation. Do I leave in TCA for the 4 weeks or place in PBS until they come to Glasgow. This is a field trip so further processing is not possible, it has to be fix or fix then buffer before the flight back to Scotland.<br> <x-tab>        </x-tab>All this because my microscopy brief was expanded. Wish it was the wallet and not just the waist line that is expanding.<br> Ian.    <br> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail:</font></html>
<< Previous Message | Next Message >>