need help....

From:Colleen Forster <cforster@tc.umn.edu>


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I received a specimen today that they would desperately like to get some
type of histology on. Here is what it is:

a vial of fluid in 10% formalin consisting of ?/CSF,blood,perhaps some
brain matter. The contents were aspirated in
a state of desperation. This is a sad case and any suggestions on
handling the specimen would be greatly appreciated.
I am wondering if it could be spun into a pellet, much like a cytology
specimen, and histgel used to create a pellet and then
process. if that would be a possibility what type of a processing
schedule would one use??

Also, 3 tubes of CSF were drawn and put into purple top tubes (EDTA). Is
there anything that I can do histologically with
an unfixed specimen in purple top tubes?? Again, the question would be
fixing the liquid, spinning etc. If you think it could
be fixed at this time  (it was drawn on May 6) how long would you fix
for ??/ And then how might you handle it from there.??

Any responses would be greatly appreciated!!

On a lighter note I am wondering if anyone do GABA staining would share
their antibody source?? The one I ahve is from Sigma,
the polyclonal and I am having trouble with background. Again, any help
would be appreciated!!

As Always,

Colleen Forster
U of MN, Dept. of Neurology
cforster@tc.umn.edu
612-626-2477

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<font size=+1>I received a specimen today that they would desperately like
to get some type of histology on. Here is what it is:</font><font size=+1></font>
<p><font size=+1>a vial of fluid in 10% formalin consisting of ?/CSF,blood,perhaps
some brain matter. The contents were aspirated in</font>
<br><font size=+1>a state of desperation. This is a sad case and any suggestions
on handling the specimen would be greatly appreciated.</font>
<br><font size=+1>I am wondering if it could be spun into a pellet, much
like a cytology specimen, and histgel used to create a pellet and then</font>
<br><font size=+1>process. if that would be a possibility what type of
a processing schedule would one use??</font><font size=+1></font>
<p><font size=+1>Also, 3 tubes of CSF were drawn and put into purple top
tubes (EDTA). Is there anything that I can do histologically with</font>
<br><font size=+1>an unfixed specimen in purple top tubes?? Again, the
question would be fixing the liquid, spinning etc. If you think it could</font>
<br><font size=+1>be fixed at this time  (it was drawn on May 6) how
long would you fix for ??/ And then how might you handle it from there.??</font><font size=+1></font>
<p><font size=+1>Any responses would be greatly appreciated!!</font><font size=+1></font>
<p><font size=+1>On a lighter note I am wondering if anyone do GABA staining
would share their antibody source?? The one I ahve is from Sigma,</font>
<br><font size=+1>the polyclonal and I am having trouble with background.
Again, any help would be appreciated!!</font><font size=+1></font>
<p><font size=+1>As Always,</font><font size=+1></font>
<p><font size=+1>Colleen Forster</font>
<br><font size=+1>U of MN, Dept. of Neurology</font>
<br><font size=+1>cforster@tc.umn.edu</font>
<br><font size=+1>612-626-2477</font></html>

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