|From:||Claire Delattre <email@example.com>|
We have a retraction problem between the muscular fiber and the connective tissue. The specimens (5mm by 7mm) are fixed in 10 % formalin overnight, then in PBS buffer until processed as follow:
-alcohol, 70%, 80%, 95%, 95%, 100%, 100%, 100% an hour for each solution
-then 2 xylene substitute an hour each,
-4 paraffin 30 minutes each.
Does anybody has experienced and fixed this kind of artefact?
Thank of in advance for your inputs.
OHSU West Campus
185th N.W. Avenue
Beaverton, Oregon 97006-3499
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