detergents in antibody diuents

From:Ron Salisbury <>

I have seen various concentrations of detergents (Triton X, Tween 20
etc.) used in primary antibody diluents and wondered if there were any
guidelines for choosing a particular concentration. Does it depend on
whether paraffin or frozen sections are used or is it more dependent
upon the type and concentration of fixative? Also, is there an effect of
detergent concentration on the ability of sections to adhere to slides?

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