cornflaking -Reply

From:Tony Henwood <>


If the artifact is predominantly over the nuclei, then it is probably caused
by partial air drying after xylene before coverslipping.

Are you sure its not caused by glycogen in the squames? This can
cause a brown atifact predominantley cytoplasm based.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> "Burnette, Andrew - MHA" <> 8/May/2001
04:20am >>>
We have a sudden appearance of "cornflaking", i.e., granular brownish
artifact obscuring cell detail in our cervical pap smears. I expect to find
some modification within the pap stainning technique that is allowing
airdrying during the staining process, but I am wondering, amongst
who have experienced this artifact, what other causes have been
More importantly, however, is how to get rid of the artifact once it does
appear. I've heard references to a glycerin technique - Could anyone
enlighten me to this or other techniques that work?  Thanks!

Andy Burnette
Section Head Anatomic Pathology
St. Joseph's Hospital, Phoenix AZ
(602) 406-3272

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of the
Childrens Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 

<< Previous Message | Next Message >>