? about Bouins fixative

From:Snobird75@aol.com


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Hello
I would like to know if there is any difference with staining results:
On paraffin sections using any one of these steps.
1. If you first fix the tissue in Bouins.

2. If you use NBF fixed cut section on a slide in Bouins in an oven for 1hr .
 
3. If you use NBF  fixed cut section on a slide in Bouins on the countertop 
    overnight.
I know all three steps will work but will the end result stain better.
I am using a elestic stain.

Thank you for any input.
Have a great weekend
Sandi Miller HT
USAMRICD Research
Maryland

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>Hello
<BR>I would like to know if there is any difference with staining results:
<BR>On paraffin sections using any one of these steps.
<BR>1. If you first fix the tissue in Bouins.
<BR>
<BR>2. If you use NBF fixed cut section on a slide in Bouins in an oven for 1hr .
<BR> 
<BR>3. If you use NBF  fixed cut section on a slide in Bouins on the countertop 
<BR>    overnight.
<BR>I know all three steps will work but will the end result stain better.
<BR>I am using a elestic stain.
<BR>
<BR>Thank you for any input.
<BR>Have a great weekend
<BR>Sandi Miller HT
<BR>USAMRICD Research
<BR>Maryland</FONT></HTML>

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