To clarify my problem...
From: | Linda Jenkins <jlinda@ces.clemson.edu> |
Hi, Jack,
The terms that we read in a procedure stating "deparaffinize and
hydrate to water" are not written in stone. For instance, my procedure,
that works beautifully for me, is:
1. Xylene -- 10 dips
2. Xylene -- 5 minutes
3. 100% ethanol -- 10 dips
4. 100% ethanol -- 1 minute
5. 95% ethanol --10 dips
6. 95% ethanol -- 1 minute
7. Distilled water
Total time is seven minutes plus 30 dips(~1.5min)=Grand Total is 8.5 minutes.
Tryit - you may like it!
Linda
*********************************
Linda Jenkins, HT
Clemson University
Department of Bioengineering
864.656.5553
**********************************
<< Previous Message | Next Message >>