Re: protocol for using gelatin
From: | "J. A. Kiernan" <jkiernan@uwo.ca> |
On Fri, 11 May 2001, frank quin wrote:
> I need to cut spinal cord tissue with Vibratome but could not do
> it well. Would you please give me some advice?
> Do you have the protocol about how to make gelatin
> for imbedding and how to fix gelatin in formaldehyde (% and how long)? =20
> I couldn’t find such information in your website. Thanks.
It should be in any manual. It doesn't much matter what you
do. Try 5% gelatin in water. cast a cube containing the
specimen. Trim it when set (fridge). Immerse in 10%
formalin in water overnight (in fridge if it's warm
enough in the lab to melt unfixed gelatin). Wash and cut
sections.
----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan
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