Re: need help....
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Dear Vinnie
My observation is in fresh smears, also while some rbcs are intact, it is
obvious from the brown fluid that formalin becomes after sitting on tissue
that some or the bulk of rbcs are lysed, this "brown" is caused by many thigs
like bile, and other fluids, but is also caused by the lysing or rbcs and the
release of hemoglobin, and bilirubin by-products of blood. So to explain why
some are intact is due to the rapid uptake of fluid into tissue and the
numbers of rbc outweigh(so to speak the amount of water).
Dana
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Dear Vinnie
<BR>My observation is in fresh smears, also while some rbcs are intact, it is
<BR>obvious from the brown fluid that formalin becomes after sitting on tissue
<BR>that some or the bulk of rbcs are lysed, this "brown" is caused by many thigs
<BR>like bile, and other fluids, but is also caused by the lysing or rbcs and the
<BR>release of hemoglobin, and bilirubin by-products of blood. So to explain why
<BR>some are intact is due to the rapid uptake of fluid into tissue and the
<BR>numbers of rbc outweigh(so to speak the amount of water).
<BR> Dana</FONT></HTML>
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