Re: need help....
| From: | Sharon Sharon <topper57@myexcel.com> |
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Try cytospinning both specimens. Any material will be on the slide.
You may want to lyse the RBC's in the first speciment first if there is
enough to interfere with the materials...sharon osborn
Colleen Forster wrote:
> I received a specimen today that they would desperately like to get
> some type of histology on. Here is what it is:
>
> a vial of fluid in 10% formalin consisting of ?/CSF,blood,perhaps some
> brain matter. The contents were aspirated in
> a state of desperation. This is a sad case and any suggestions on
> handling the specimen would be greatly appreciated.
> I am wondering if it could be spun into a pellet, much like a cytology
> specimen, and histgel used to create a pellet and then
> process. if that would be a possibility what type of a processing
> schedule would one use??
>
> Also, 3 tubes of CSF were drawn and put into purple top tubes (EDTA).
> Is there anything that I can do histologically with
> an unfixed specimen in purple top tubes?? Again, the question would be
> fixing the liquid, spinning etc. If you think it could
> be fixed at this time (it was drawn on May 6) how long would you fix
> for ??/ And then how might you handle it from there.??
>
> Any responses would be greatly appreciated!!
>
> On a lighter note I am wondering if anyone do GABA staining would
> share their antibody source?? The one I ahve is from Sigma,
> the polyclonal and I am having trouble with background. Again, any
> help would be appreciated!!
>
> As Always,
>
> Colleen Forster
> U of MN, Dept. of Neurology
> cforster@tc.umn.edu
> 612-626-2477
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Try cytospinning both specimens. Any material will be on the slide.
You may want to lyse the RBC's in the first speciment first if there is
enough to interfere with the materials...sharon osborn
<p>Colleen Forster wrote:
<blockquote TYPE=CITE><font size=+1>I received a specimen today that they
would desperately like to get some type of histology on. Here is what it
is:</font>
<p><font size=+1>a vial of fluid in 10% formalin consisting of ?/CSF,blood,perhaps
some brain matter. The contents were aspirated in</font>
<br><font size=+1>a state of desperation. This is a sad case and any suggestions
on handling the specimen would be greatly appreciated.</font>
<br><font size=+1>I am wondering if it could be spun into a pellet, much
like a cytology specimen, and histgel used to create a pellet and then</font>
<br><font size=+1>process. if that would be a possibility what type of
a processing schedule would one use??</font>
<p><font size=+1>Also, 3 tubes of CSF were drawn and put into purple top
tubes (EDTA). Is there anything that I can do histologically with</font>
<br><font size=+1>an unfixed specimen in purple top tubes?? Again, the
question would be fixing the liquid, spinning etc. If you think it could</font>
<br><font size=+1>be fixed at this time (it was drawn on May 6) how
long would you fix for ??/ And then how might you handle it from there.??</font>
<p><font size=+1>Any responses would be greatly appreciated!!</font>
<p><font size=+1>On a lighter note I am wondering if anyone do GABA staining
would share their antibody source?? The one I ahve is from Sigma,</font>
<br><font size=+1>the polyclonal and I am having trouble with background.
Again, any help would be appreciated!!</font>
<p><font size=+1>As Always,</font>
<p><font size=+1>Colleen Forster</font>
<br><font size=+1>U of MN, Dept. of Neurology</font>
<br><font size=+1>cforster@tc.umn.edu</font>
<br><font size=+1>612-626-2477</font></blockquote>
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