Re: need help.... -Reply

From:Tony Henwood <AnthonyH@chw.edu.au>

Dear All,

Also smears are air dried prior to formalin fixation (usually). Possibly this
has something to do with the lysing of rbc.

This is the basis of the smear rehydration technique of  Chan & Kung
(Am J Clin Pathol 1988:89:30-34).

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> <DDittus787@aol.com> 12/May/2001 08:53am >>>
Dear Vinnie
My observation is in fresh smears, also while some rbcs are intact, it is 
obvious from the brown fluid that formalin becomes after sitting on tissue

that some or the bulk of rbcs are lysed, this "brown" is caused by many
thigs 
like bile, and other fluids, but is also caused by the lysing or rbcs and the 
release of hemoglobin, and bilirubin by-products of blood. So to explain
why 
some are intact is due to the rapid uptake of fluid into tissue and the 
numbers of rbc outweigh(so to speak the amount of water).
                                           Dana



**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of the
Childrens Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************



<< Previous Message | Next Message >>