Re: need help.... -Reply

From:Tony Henwood <>

Dear All,

Also smears are air dried prior to formalin fixation (usually). Possibly this
has something to do with the lysing of rbc.

This is the basis of the smear rehydration technique of  Chan & Kung
(Am J Clin Pathol 1988:89:30-34).

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> <> 12/May/2001 08:53am >>>
Dear Vinnie
My observation is in fresh smears, also while some rbcs are intact, it is 
obvious from the brown fluid that formalin becomes after sitting on tissue

that some or the bulk of rbcs are lysed, this "brown" is caused by many
like bile, and other fluids, but is also caused by the lysing or rbcs and the 
release of hemoglobin, and bilirubin by-products of blood. So to explain
some are intact is due to the rapid uptake of fluid into tissue and the 
numbers of rbc outweigh(so to speak the amount of water).

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