Hi,
    Our antibody (from DAKO) is kind of old so it wouldn't surprise me at
all if you get a different dilution than we have now. After peroxidase
blocking, we pretreat with tripsin for 15 min, then incubate for 30 min at
room temp. at 1:10,000. (The dilution is why the antibody is old, it will
last forever at this rate) Our detection kit is LSAB+ and DAB chromogen.
hope this helps
Amos Brooks
----- Original Message -----
From: "Aneeta Patel" <apatel@usuhs.mil>
To: <Histonet@pathology.swmed.edu>
Sent: Wednesday, May 02, 2001 12:21 PM
Subject: factor 8


hi  all
I am trying to find out if anyone has done factor 8 immunostaining on
parafiin embedded tissues and if so can you kindly share the details of
method including ag retrival,thanks