We have used Tween 20 in our antibody diluent at a concentration  of 0.05%
without any problems on both paraffin and frozen sections.

Bob Quilty
Dept of Neuropathology
Frenchay Hospital
Bristol  UK

----- Original Message -----
From: Ron Salisbury <ronald2@uakron.edu>
To: Histonet <histonet@pathology.swmed.edu>
Sent: Tuesday, May 01, 2001 7:06 PM
Subject: detergents in antibody diuents


> I have seen various concentrations of detergents (Triton X, Tween 20
> etc.) used in primary antibody diluents and wondered if there were any
> guidelines for choosing a particular concentration. Does it depend on
> whether paraffin or frozen sections are used or is it more dependent
> upon the type and concentration of fixative? Also, is there an effect of
> detergent concentration on the ability of sections to adhere to slides?
>
>